In addition to reducing grain yield and quality, the harvested grains are often contaminated with mycotoxins that are a major health and food safety concern due to their toxicity to humans and other animals. įunding: This work was supported by AAP PSPE 2012 (Appel à proposition Pour et Sur le Plan Ecophytro de Juin 2012) Competing interests: The authors have declared that no competing interests exits.įusarium head blight (FHB) is a major cereal disease causing severe damage to crops worldwide including Europe.
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Metabarcoding data are deposited in the European Nucleotide Archive (ENA) (accession number: PRJEB27158). Sanger DNA sequences are deposited in R-syst::fungi database ( ). This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.ĭata Availability: All relevant data are within the paper and its Supporting Information file. Received: OctoAccepted: NovemPublished: January 11, 2019Ĭopyright: © 2019 Boutigny et al. PLoS ONE 14(1):Įditor: Ulrich Melcher, Oklahoma State University, UNITED STATES (2019) Metabarcoding targeting the EF1 alpha region to assess Fusarium diversity on cereals.
Information on the distribution and prevalence of the different Fusarium species in a given geographical area, and in response to various environmental factors, is of great interest for managing the disease and predicting mycotoxin contamination risks.Ĭitation: Boutigny A-L, Gautier A, Basler R, Dauthieux F, Leite S, Valade R, et al. This new experimental approach may be used to show changes in the composition of the Fusarium complex or to detect the emergence of new Fusarium species as far as the EF1α sequence of these species show a sufficient amount of polymorphism in the portion of sequence analyzed. It provides a more exhaustive picture of the Fusarium community than the currently used techniques based on isolation or species-specific PCR detection.
This new set of primers enables the assessment of Fusarium diversity by high-throughput sequencing on cereal samples. Up to 17 different Fusarium species were detected in field samples of barley, durum and soft wheat harvested in France. The method enabled the detection of as few as one single Fusarium-infected grain in 10,000.
Fusarium species were retrieved from mock communities and good reproducibility between different runs or PCR cycle numbers was be observed. The PCR amplification parameters and bioinformatic pipeline were optimized with mock and artificially infected grain communities and further tested on 65 field samples. In this study, using a new set of primers targeting the EF1α gene, the diversity of Fusarium species on cereals was evaluated using Illumina high-throughput sequencing. Accurate and exhaustive identification of Fusarium species in planta is therefore of great importance.
The Fusarium species involved in FHB differ in their pathogenicity, ability to produce mycotoxins, and fungicide sensitivity. These mycotoxins constitute a significant food safety concern as they have health implications in both humans and animals. In addition, Fusarium species can release toxic secondary metabolites. These species vary in importance depending on climatic conditions, agronomic factors or host genotype. Fusarium head blight (FHB) is a major cereal disease caused by a complex of Fusarium species.